P.G. DIPLOMA EXAMINATION, MAY 2011
Biotechnology
Paper III — PLANT AND ANIMAL TISSUE CULTURE AND GENETIC ENGINEERING
Time : Three hours Maximum : 100 marks
Answer any FIVE questions.
All questions carry equal marks.
1. Describe the methods of sterilizations of media.
2. Write an account on Bergman's plating techniques.
3. Describe how the haploids are produced and their importance in plant tissue culture.
4. Write an account on the isolation of protoplast, its culture and fusion techniques.
5. Describe the various constituents of culture medium.
6. Write an account on the techniques and types of mammalian cell cultures.
7. Describe stem cell culture techniques and its applications.
8. Write an account on in-vitro fertilizations and embryo transfer.
9. Describe how the enzymes are used in genetic engineering.
10. Write an account on gene therapy and its applications in biotechnology.
Biotechnology
Paper III — PLANT AND ANIMAL TISSUE CULTURE AND GENETIC ENGINEERING
Time : Three hours Maximum : 100 marks
Answer any FIVE questions.
All questions carry equal marks.
1. Describe the methods of sterilizations of media.
2. Write an account on Bergman's plating techniques.
3. Describe how the haploids are produced and their importance in plant tissue culture.
4. Write an account on the isolation of protoplast, its culture and fusion techniques.
5. Describe the various constituents of culture medium.
6. Write an account on the techniques and types of mammalian cell cultures.
7. Describe stem cell culture techniques and its applications.
8. Write an account on in-vitro fertilizations and embryo transfer.
9. Describe how the enzymes are used in genetic engineering.
10. Write an account on gene therapy and its applications in biotechnology.
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